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mab221  (R&D Systems)


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    Structured Review

    R&D Systems mab221
    Mab221, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mab221/Human+PDGF-AA+Antibody/pm36482075-203-110-116
    Average 90 stars, based on 4 article reviews
    mab221 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration.
    Article Snippet: For the screening, the proteins; HGF (294-HG-025/CF, RnD systems), VEGF (293-VE-010/CF, RnD systems), BMP4 (314-BP-010/CF, RnD systems) CXCL12, (350-NS-010/CF, RnD systems), IL-6 (7270-IL-025/CF, RnD systems), FGF2 (ab9596, Abcam), PDGF-AA (221-AA-010, RnD systems), KGF (251-KG010/CF, RnD systems), and TGF beta 1 (7754-BH-025/CF, RnD systems) were dissolved at a concentration of 10 μg/ml in PBS (1891014, Thermo Fisher), Tween 20 (P1379, Sigma-Aldrich,) and 10% BSA (A2153, Sigma-Aldrich), and 100 μl of the solution was added to the slide and incubated in a humified chamber for 60 min. .. The slides were rinsed in PBST with 1% BSA for 3 min and then in deionized water for 3 min. Antibodies towards the protein were dissolved in PBST 10% BSA according to the manufacturer’s recommended concentration, added to the slide and incubated in a humified chamber for 60 min (HGF Ab, MAB694, 0.4 μg/ml, Mouse anti Human, RnD systems) (VEGF Ab, MAB293, 0.4 μg/ml, Mouse anti Human, RnD systems) (BMP4 Ab, MAB7571, 0.4 μg/ml, Mouse anti Human, RnD systems) (CXCL12 Ab, MAB350, 0.4 μg/ml, Mouse ant Human, RnD Systems) (IL-6 Ab, MAB2063, 0.4 μg/ml, Mouse ant Human, RnD systems) (FGF2 Ab, ab92337, 0.5 μg/ml, Rabbit anti Human, Abcam) (PDGF-AA Ab, MAB221, 0.4 μg/ml, Mouse anti Human, RnD systems) (KGF Ab, MAB251, 0.4 μg/ml, Mouse anti Human, RnD systems) (TGF beta 1 Ab, MAB240, 0.4 μg/ml, Mouse anti Human, RnD systems). .. The slide was washed with PBST 1% BSA and deionized water for 3 min. A secondary antibody conjugated to the fluorochrome Alexa fluor Plus 647 (IgG Alexa 647 plus, A32728, 1/1000, Goat anti Mouse, Thermo Fisher or IgG Alexa 647 plus, A32733, 1/1000, Goat anti Rabbit, Thermo Fisher) was added at a 1/1000 dilution in PBST 10% BSA and incubated in a humified chamber for 60 min.

    Article Title: Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration
    Article Snippet: For the screening, the proteins; HGF (294-HG-025/CF, RnD systems), VEGF (293-VE-010/CF, RnD systems), BMP4 (314-BP-010/CF, RnD systems) CXCL12, (350-NS-010/CF, RnD systems), IL-6 (7270-IL-025/CF, RnD systems), FGF2 (ab9596, Abcam), PDGF-AA (221-AA-010, RnD systems), KGF (251-KG-010/CF, RnD systems), and TGF beta 1 (7754-BH-025/CF, RnD systems) were dissolved at a concentration of 10 μg/ml in PBS (1891014, Thermo Fisher), Tween 20 (P1379, Sigma-Aldrich,) and 10% BSA (A2153, Sigma-Aldrich), and 100 μl of the solution was added to the slide and incubated in a humified chamber for 60 min. .. The slides were rinsed in PBST with 1% BSA for 3 min and then in deionized water for 3 min. Antibodies towards the protein were dissolved in PBST 10% BSA according to the manufacturer’s recommended concentration, added to the slide and incubated in a humified chamber for 60 min (HGF Ab, MAB694, 0.4 μg/ml, Mouse anti Human, RnD systems) (VEGF Ab, MAB293, 0.4 μg/ml, Mouse anti Human, RnD systems) (BMP4 Ab, MAB7571, 0.4 μg/ml, Mouse anti Human, RnD systems) (CXCL12 Ab, MAB350, 0.4 μg/ml, Mouse ant Human, RnD Systems) (IL-6 Ab, MAB2063, 0.4 μg/ml, Mouse ant Human, RnD systems) (FGF2 Ab, ab92337, 0.5 μg/ml, Rabbit anti Human, Abcam) (PDGF-AA Ab, MAB221, 0.4 μg/ml, Mouse anti Human, RnD systems) (KGF Ab, MAB251, 0.4 μg/ml, Mouse anti Human, RnD systems) (TGF beta 1 Ab, MAB240, 0.4 μg/ml, Mouse anti Human, RnD systems). .. The slide was washed with PBST 1% BSA and deionized water for 3 min. A secondary antibody conjugated to the fluorochrome Alexa fluor Plus 647 (IgG Alexa 647 plus, A32728, 1/1000, Goat anti Mouse, Thermo Fisher or IgG Alexa 647 plus, A32733, 1/1000, Goat anti Rabbit, Thermo Fisher) was added at a 1/1000 dilution in PBST 10% BSA and incubated in a humified chamber for 60 min.

    Incubation:

    Article Title: Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration.
    Article Snippet: For the screening, the proteins; HGF (294-HG-025/CF, RnD systems), VEGF (293-VE-010/CF, RnD systems), BMP4 (314-BP-010/CF, RnD systems) CXCL12, (350-NS-010/CF, RnD systems), IL-6 (7270-IL-025/CF, RnD systems), FGF2 (ab9596, Abcam), PDGF-AA (221-AA-010, RnD systems), KGF (251-KG010/CF, RnD systems), and TGF beta 1 (7754-BH-025/CF, RnD systems) were dissolved at a concentration of 10 μg/ml in PBS (1891014, Thermo Fisher), Tween 20 (P1379, Sigma-Aldrich,) and 10% BSA (A2153, Sigma-Aldrich), and 100 μl of the solution was added to the slide and incubated in a humified chamber for 60 min. .. The slides were rinsed in PBST with 1% BSA for 3 min and then in deionized water for 3 min. Antibodies towards the protein were dissolved in PBST 10% BSA according to the manufacturer’s recommended concentration, added to the slide and incubated in a humified chamber for 60 min (HGF Ab, MAB694, 0.4 μg/ml, Mouse anti Human, RnD systems) (VEGF Ab, MAB293, 0.4 μg/ml, Mouse anti Human, RnD systems) (BMP4 Ab, MAB7571, 0.4 μg/ml, Mouse anti Human, RnD systems) (CXCL12 Ab, MAB350, 0.4 μg/ml, Mouse ant Human, RnD Systems) (IL-6 Ab, MAB2063, 0.4 μg/ml, Mouse ant Human, RnD systems) (FGF2 Ab, ab92337, 0.5 μg/ml, Rabbit anti Human, Abcam) (PDGF-AA Ab, MAB221, 0.4 μg/ml, Mouse anti Human, RnD systems) (KGF Ab, MAB251, 0.4 μg/ml, Mouse anti Human, RnD systems) (TGF beta 1 Ab, MAB240, 0.4 μg/ml, Mouse anti Human, RnD systems). .. The slide was washed with PBST 1% BSA and deionized water for 3 min. A secondary antibody conjugated to the fluorochrome Alexa fluor Plus 647 (IgG Alexa 647 plus, A32728, 1/1000, Goat anti Mouse, Thermo Fisher or IgG Alexa 647 plus, A32733, 1/1000, Goat anti Rabbit, Thermo Fisher) was added at a 1/1000 dilution in PBST 10% BSA and incubated in a humified chamber for 60 min.

    Article Title: Controlled release of growth factors using synthetic glycosaminoglycans in a modular macroporous scaffold for tissue regeneration
    Article Snippet: For the screening, the proteins; HGF (294-HG-025/CF, RnD systems), VEGF (293-VE-010/CF, RnD systems), BMP4 (314-BP-010/CF, RnD systems) CXCL12, (350-NS-010/CF, RnD systems), IL-6 (7270-IL-025/CF, RnD systems), FGF2 (ab9596, Abcam), PDGF-AA (221-AA-010, RnD systems), KGF (251-KG-010/CF, RnD systems), and TGF beta 1 (7754-BH-025/CF, RnD systems) were dissolved at a concentration of 10 μg/ml in PBS (1891014, Thermo Fisher), Tween 20 (P1379, Sigma-Aldrich,) and 10% BSA (A2153, Sigma-Aldrich), and 100 μl of the solution was added to the slide and incubated in a humified chamber for 60 min. .. The slides were rinsed in PBST with 1% BSA for 3 min and then in deionized water for 3 min. Antibodies towards the protein were dissolved in PBST 10% BSA according to the manufacturer’s recommended concentration, added to the slide and incubated in a humified chamber for 60 min (HGF Ab, MAB694, 0.4 μg/ml, Mouse anti Human, RnD systems) (VEGF Ab, MAB293, 0.4 μg/ml, Mouse anti Human, RnD systems) (BMP4 Ab, MAB7571, 0.4 μg/ml, Mouse anti Human, RnD systems) (CXCL12 Ab, MAB350, 0.4 μg/ml, Mouse ant Human, RnD Systems) (IL-6 Ab, MAB2063, 0.4 μg/ml, Mouse ant Human, RnD systems) (FGF2 Ab, ab92337, 0.5 μg/ml, Rabbit anti Human, Abcam) (PDGF-AA Ab, MAB221, 0.4 μg/ml, Mouse anti Human, RnD systems) (KGF Ab, MAB251, 0.4 μg/ml, Mouse anti Human, RnD systems) (TGF beta 1 Ab, MAB240, 0.4 μg/ml, Mouse anti Human, RnD systems). .. The slide was washed with PBST 1% BSA and deionized water for 3 min. A secondary antibody conjugated to the fluorochrome Alexa fluor Plus 647 (IgG Alexa 647 plus, A32728, 1/1000, Goat anti Mouse, Thermo Fisher or IgG Alexa 647 plus, A32733, 1/1000, Goat anti Rabbit, Thermo Fisher) was added at a 1/1000 dilution in PBST 10% BSA and incubated in a humified chamber for 60 min.



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